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Published:
Journal of Analytical Toxicology,
ISSN 0146-4760,
Volume 32, Number 4, May,
pp.292-297
Novel Automated
Extraction Method for Quantitative Analysis of Urinary 11-nor-Δ9-Tetrahydrocannabinol-9-Carboxylic
Acid (THC-COOH)
Shanlin Fu and John Lewis
Toxicology Unit, Pacific Laboratory Medicine Services, Northern
Sydney Central Coast Area Health Services, New South Wales, Australia
2113
An automated extraction method for extracting
the major urinary metabolite of cannabis, 11-nor-Δ9-tetrahydrocannabinol-9-carboxylic
acid (THC-COOH) was developed on the four-probe Gilson ASPEC
XL4™ solid-phase extraction (SPE) system. The method works
on liquid–liquid extraction principles but does not require
the use of SPE cartridges. The limits of detection and quantitation
and the upper limit of linearity (ULOL) of the developed method
were found to be 1, 2, and 1500 ng/mL, respectively. There was
no detectable carry over after 10,000 ng/mL analyte. For a batch
of 76 samples, the process uses less than 100 mL methanol, 450
mL extracting solvent hexane/ethyl acetate (5:1, v/v) and 1 L
rinsing solvent, 30% methanol in water. The automated extraction
process takes 5 h to complete. Precision and accuracy of the
method are comparable to both manual liquid–liquid extraction
and automated SPE methods. The method has proven to be a simple,
speedy, and economical alternative to the currently popular automated
SPE method for the quantitative analysis of urinary THC-COOH.
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