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Published:
Journal of Analytical Toxicology,
ISSN 0146-4760,
Volume 32, Number 6, July/August,
pp.417-421
The
Detection and Quantitative Analysis of the Psychoactive Component
of Salvia divinorum, Salvinorin A, in Human Biological Fluids Using
Liquid Chromatography–Mass Spectrometry
Pamela C. McDonough1, Justin M.
Holler1, Shawn P. Vorce1,
Thomas Z. Bosy2, Joseph Magluilo, Jr.1, and
Marilyn R. Past1
1Division of Forensic Toxicology, The Armed Forces Medical Examiner
System, Armed Forces Institute of Pathology, 1413 Research Boulevard,
Building 102, Rockville, Maryland 20850-3125, and
2Navy Drug Screening
Laboratory, Bldg. H2033, Naval Air Station, Jacksonville, Florida
32212
Salvia divinorum, a member of the mint plant family,
has hallucinogenic properties that have become increasingly sought
after by recreational drug users. The main psychoactive component,
salvinorin A, has potency comparable to lysergic acid diethylamide.
Though still legal to possess in most of the United States and
much of Europe, little is known regarding the compound’s
long-term health effects, addiction liability, and pharmacokinetics.
Limited data are available in the scientific literature, and
few analytical methods are published for the detection in human
biological fluids. These factors contribute to the unfamiliarity
of the compound and complicate the method development process
necessary to accommodate special requested testing for salvinorin
A. A sensitive analytical method for the detection and quantitation
of salvinorin A in human biological fluids was developed and
validated to resolve analytical shortcomings. The method utilizes
a solid-phase extraction technique coupled with liquid chromatography–electrospray
ionization mass spectrometry operated in selected ion monitoring
mode. The assay has a linear range of 5.0–100 ng/mL with
a correlation coefficient of 0.997. The limit of detection and
limit of quantitation were experimentally determined as 2.5 and
5.0 ng/mL, respectively. The method has been applied to blood
and urine samples successfully and can be used to detect the
presence of salvinorin A in forensic testing.
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