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Published:
Journal of Analytical Toxicology,
ISSN 0146-4760,
Volume 32, Number 1, January/February,
pp.92-98
Validation and Application of a GC–MS
Method for Determining Soman Concentration in Rat Plasma Following
Low-Level Vapor Exposure
Julie A. Renner[1], Paul A. Dabisch[2], Ronald A. Evans[2],
Jeffrey M. McGuire[2], Allison L. Totura[1], Edward M. Jakubowski[2],
and Sandra A. Thomson[2]
[1]SAIC, Gunpowder Branch, Aberdeen Proving Ground, Maryland
21010-0068 and
[2]U.S. Army Edgewood Chemical Biological Center,
Aberdeen Proving Ground, Maryland 21010-5424
A method for determining the chemical warfare
agent soman (GD) in rat plasma has been validated and applied
to low-level inhalation exposure studies currently being conducted.
This method utilizes a fluoride ion-based regeneration assay
with isotope dilution followed by large volume injection gas
chromatography with ammonia chemical ionization mass spectrometric
detection. Following sample preparation by solid phase extraction,
chromatographic separation was achieved using a 14% cyanopropylphenyl/86%
dimethyl polysiloxane capillary column with a total run time
of 18.16 min. Soman and the deuterated isotope (2H4-soman) internal
standard were detected using the selected ion monitoring mode
and quantitated using the ammonia adduction ratio of m/z ions
200/204. A reproducible linear relationship was obtained for
the quantitative concentration range of 10 pg on-column to 1000
pg on-column (r2 = 0.9995) for standards in ethyl acetate with
a detection limit of 5.65 pg on-column, and an average recovery
of 93% in plasma. This sensitive method was successfully applied
to the analysis of soman in rat plasma immediately post-exposure,
resulting in the construction of dose-response plots.
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