| |
Published:
Journal of Analytical Toxicology,
ISSN 0146-4760,
Volume 31, Number 8, October,
pp.442-446
Achieving Proposed Federal Concentrations
using Reduced Specimen Volume for the Extraction of Amphetamines
from Oral Fluid
Christine Moore, Cynthia Coulter,
and Katherine Crompton
Immunalysis Corporation, 829 Towne Center Drive, Pomona, California
91767
This article details the rapid extraction of amphetamines
from oral fluid using low specimen volume, low sorbent bed mass,
and fast gas chromatography with mass selective detection. The
collection of a known amount of sample volume coupled with high
percentage recovery of drug from the collection pad has allowed
oral fluid to be increasingly employed as the specimen of choice
for the detection of drug use in various applications. Additionally,
low specimen volume for confirmation is required, so that adequate
test volume remains for second analysis in case of batch failure
or for testing at a different laboratory facility. The extracts
were prepared using low bed mass sorbent, so less conditioning,
washing, and elution solvent further reduced the overall cost
of sample preparation. The limits of quantitation for the assay
were 25 ng/mL; the intraday precision of the assays (n = 5) ranged
from 0.3 to 3.99%; interday precision ranged from 0.72 to 4.6%
for the amphetamine class. The percentage recovery of the drugs
from the collection pads ranged from 85.4 to 89.1% (n = 6). The
process lends itself to widely available automated processing
instrumentation and significantly increases the efficiency of
laboratories providing high-volume oral fluid analysis for drugs
of abuse.
Reproduction
of editorial content of this journal is prohibited without publisher’s
permission.
This
article is available in its entirety by fax for $40.00 each.
Visa, MasterCard and AMEX accepted.
To
order electronically click here
or call: 847-647-2900 ext. 1323
or fax request to: 847-647-1155.
To order multiple copies click here.
Please indicate JAT
volume and issue along with page numbers. |
|
Home | Subscribe
| Current Issue | Back Issues
| Search | Advertise | Other Publications
| |
